Quiz 3  (H & J Chapters 9 & 10)

The quiz is due class time on the Monday.  Print this sheet; go through the book carefully to answer questions. 
Then go to D2L to answer the questions. 

1. A gene that normally "on" but is repressed when an aporepressor combines with a co-repressor is an example of (p319; also see Fig. 9.1):
    a)  inducible transcription, which is a version of negative regulation.
    b)  repressible transcription, which is a version of negative regulation.
    c)  inducible transcription, which is a version of positive regulation. 
    d)  repressible transcription, which is a version of positive regulation. 

2. The lac operon is repressed when a repressor protein binds to which element (p 322, Fig. 9.4)?
    a) i
    b) p
    c) o
    d) z

3.  Even if there is no repressor, the lac operon cannot efficiently transcribe mRNA without the presence of what (p325)?
    a) cAMP-CRP
    b) glucose
    c) tryptophan
    d) ß-galactosidase

4. Attenuation of the trp operon is caused by (p. 328-9; read carefully, b isn't the right answer):
    a)  the leader polypeptide acts as a corepressor.
    b)  insufficient tryptophan causes the ribosomes to fall off. 
    c)  the formation of a stem and loop in the mRNA.
    d)  cAMP-CRP complexes. 

5. A TATA-box-binding protein is one component of (pp 336-7):
    a) transcriptional activators.
    b) basal transcription factors.
    c) TBP-associated factors.
    d) Pol II holoenzyme.

6. Which of these vectors is capable of holding the largest insert (p 362)?
    a)  Plasmid
    b)  Bacteriophage
    c)  Cosmid
    d)  BAC

7. A DNA molecule that is artificially copied from an mRNA is called a(n) (p365):
    a) mDNA
    b) snDNA
    c) cDNA
    d) ddDNA

8. A procedure where the sequence is known first and mutations are induced to determine the function of the sequence is called (p 377):
    a) inductive genetics.
    b) deductive genetics.
    c) molecular genetics.
    d) reverse genetics.

9. An important vector to transform plants is (p 379):
    a)  retroviruses
    b)  phages
    c)  Ti plasmids
    d)  Hfr cells

10. Transformation rescue is used to (pp 381):
    a) determine the minimal amount of a DNA sequence is necessary for full functioning of a gene.
    b) create bigger fish.
    c) cause Drosophila to change from red to white eyes.
    d) prevent disease. 

Carefully read The Human Connection: X-ing Out Gene Activity on p. 323 and answer the following questions. 
Caution:  I am looking only for the one answer that is indicated in the passage.  I reserve the right to be nit-picky on these. 

11. Evidence from XO individuals indicate that:
    a) X chromosomes are randomly inactivated.
    b) toroiseshell cats are females.
    c) one X is adequate for normal development.
    d) inactivation takes place early in development.

12. How do marsupials differ from other mammals?
    a) They do not have X-inactivation.
    b) They have XO females.
    c) Inactivation is not random.
    d) Inactivation takes place late in development.

13. If a chromosome is called heteropyknotic, that means it is:
    a) an X chromosome.
    b) a deeply staining chromosome.
    c) a paternal chromosome.
    d) heterozygous. 

14. The concept that cells can correct the level of gene expression on X chromosomes, so that females do not have twice as many gene products as males is called:
    a) mozaic phenotyping.
    b) X-ing out.
    c) sex-linked genes.
    d) dosage compensation.

15. The evidence that X chromosomes are randomly inactivated in females is that:
    a) in marsupials, only the paternally derived X is inactivated.
    b) sex-linked coat color traits cause mottling.
    c) every cell has a heteropyknotic chromosome.
    d) fifteen percent of the genes are still active.