SDSU Biol 645: MicroImaging Techniques; Part 1 Fall 2009 Assignments and Grading

1) Photoshop Project:

1. Part A (Due before Photoshop Lab)
   1. Copy the "Biol645Class2009A" file from the course computer (desktop folder: Biol645Assignments/PhotoShop/PartA) onto your USB flash drive
   2. Adjust the image size (in the menu-bar: Image/ImageSize) such that it is approximately 10cm in width with a resolution of 200 pixels/cm
   3. Using a red color (H=349 degress, S=99%, B=80%, R=203, G=3 and B=39) label the photograph with your name (use an arial, bold, 9 point font). This can be accomplished using the "horizontal-type tool) (T) in the Photoshop tool-bar.  Note that this label is added as a layer to your image.  If the "layers floating palette" is not visible on your screen, select "Window" in the menu-bar and then select "layers" in the drop-down menu.  The "layer floating palette" should then appear in the right side of your screen.
   4. Draw an arrow from your name to your head.  The arrow feature is part of the "line tool" in the tool-bar. When you select this tool, a floating palette for the line tool will appear below the menu-bar.  The arrow options are available in a drop-down menu in this floating palette. To activate the arrow select either "end" or "beginning" (depending on your preference).  Set the width at 300%, the length at 600% and the concavity at 20%.  Also, set the weight of the arrow at 14 px.  To create the arrow, simply select the spot for the beginning or end of the arrow (depending on your preference) and then drag the line to the other spot (beginning or ending spot).   Note that the arrow is also added added as a layer to your image.    
   5. Save your image with your name as the file-name using the "Photoshop" file format.  To do this select "File" in the the "Save As" feature in the "File" menu bar.  When the file has more than one layer, the default file format is ".PSD" (i.e. the Photoshope format).
   6. Also save the file as a "JPG" (jpeg) format.  To do this, you will first need to merge the layers.  This is accomplished in the "Layer" menu-bar (i.e.merge layers).  Then when you tell the program to "Save As", you can select "JPG" as an option.
   7. Copy your PSD and the JPG files into the desktop folder: Biol645Assignments/PhotoShop/PartA/StudentFiles.
2. Part B (Due Sept. 15 @5PM)
   1. Create another PSD file representing the merge of the "Biol645Class2009A" file and the file of the photo from the missing student
   2. Make the image look as "pretty" as possible
   3.  Add the following information to the "file information" section
      1. Your name
      2.  Date
   4. Copy your PSD file into the desktop folder: Biol645Assignments/PhotoShop/PartB/StudentFolders.

2) IX70 Phase Contrast Project (all TIFF images added to "assignment folder"

1. Tissue Slide with Brightfield Microscopy (Rat Small Intestine) include scale
   1. Cells in the epithelium (along the surface) using the 10X objective
   2. Cells in the epithelium (along the surface) using the 400Xobjective
2. Cell Chamber Slide (Intestinal cells in culture) include scale
   1. Cells with phase using the 10X objective
   2. Cells with brightfield using the 10X objective
   3. Cells with phase using the 40X objective
   4. Cells with brightfield using the 40X objective

Capture the original photos for the slide specimen with the DP-70 camera using the upright (IX-70) microscope.  Capture them at a resolution of 4080X3072 pixels/inch.  Save all of the files as TIF files in your own folder within the "Biol645Assignment/IX70" folder on the desktop.  Include the tissue name and magnification in the filename.  Using Photoshop, adjust the resolution of these files to 400 pixels/inch with an image size width approximating 4 inches. You should add the magnification value as a label.  Save this file again as a PSD image.

3) Tour of ADRDL Histopath Lab -
Observe Histopath Lab demonstration; no outside assignment

4) Cryostat Sectioning Project:
Observe cryostat demonstration, and cut at least one cryosection with the Leica CM1850 UV Cryostat

1. Blood Photographs:
   1. 1000X: (100X objective):
2. Tick Photograph:
   1. 40X (4X objective):
   2. 100X: (10X objective):
3. Cell Culture Slide (FluoCells® prepared slide #1 *BPAE cells with MitoTracker® Red CMXRos, Alexa Fluor® 488 phalloidin, DAPI)
   1. PSD combined (leave as 3 layers) image of cells stained with MitoTracker® Red CMXRos, Alexa Fluor® 488 phalloidin, and DAPI (use all 3 dichroic cube set); photograph only with the 40X objective; include scale.
4. Mosquito Photograph: (with Stereo-microscope)
   1. Low Magnification:
   2. High Magnification:
5. Screw Photograph: (with Stereo-microscope)
   1. Low Magnification:
   2. High Magnification:

6) Paraffin Sectioning/Staining Project:
 

Last Update: 22-Oct-09
Published by Department of Biology & Microbiology, South Dakota State University
E-mail: michael.hildreth@sdstate.edu