SDSU Biol 692: Microimaging Techniques; Part 1
Fall 2009
Course Schedule


Lectures: Tuesday 4-5PM; Lab: 2 hrs. on Wed. and/or Thurs. TBA

Sept. 1, Tues: Lect #1 Course Introduction
Lab #1 Introduction to FGCF
Sept. 8 Tues: Lect #2 Introduction to Digital Photography and Photoshop
Lab #2 Work on Photoshop Project
Sept. 15, Tues: Lect #3 Olympus IX70 Inverted Brightfield and Phase Microscopy
Lab #3 Work on IX70 Digital Image Project Involving Cells Growing in Culture
Sept. 22, Tues: Lect #4 Principles of Tissue Preparation & Microtomy
Lab #4 Tour of Animal Diagnostic Histopath Lab and Submit Tissue Samples
Sept. 29, Tues: Lect #5 Cryostat Sectioning and Introduction to Staining
Lab #5 Cut Cryosections
Oct. 6, Tues: Lect #6 Olympus AX70 Upright and Fluorescence Microscopy
Lab #6 Work on AX70 Digital Image Project involving Fluorescent Control Slides
Oct. 13, Tues: Lect #7 Immunohistochemistry Concepts; Preparation of Cytospin Concentrated Cells
Lab #6 Immunohistochemistry Project
Oct. 20, Tues: Lect #8 Paraffin Techniques: Sectioning and Staining
Lab #8 Time for Sectioning and Staining Paraffin Sections
Oct. 23 Midterm
Oct. 27, Tues: Lect #9 Evaluation Week; Discussion of Assignments
Lab #8 Individual Meeting with Students to Evaluation Assignments
Nov. 3, Tues: Lect #10 Image Analysis Overview; ImagePro Introduction
Lab #9 ImagePro Project
Nov. 10, Tues: Lect #11 Image Analysis: part 2
Lab #10 No lab due to Veterans Day
Nov. 17, Tues: Lect #12 In situ Hybridization Concepts
Lab #11 In situ Hybridization Demonstration/Project
Nov. 24, Tues: Lect #13 Introduction to Confocal Microscope; Fluoview FV300 #1: Basics Use
Lab No labs due to Thanksgiving Holiday
Dec. 1, Tues: Lect #14 Fluoview FV300 #2 Advanced Use
Lab #12 Laser Confocal Project
Dec. 8: Tues: Lect. #15 Evaluation Week; Discussion of Assignments
Lab #12 Individual Meeting with Students to Evaluation Assignments
Dec. 11 Last Day of Classes 
Dec. 14-18, Monday-Friday Final Exam Week
Dec. 23, Wednesday Grades due in Registrar's Office no later than 5:00PM 

Last Update: 27-Aug-09
Published by Department of Biology & Microbiology, South Dakota State University
E-mail: michael.hildreth@sdstate.edu