SDSU Biol 645: Microimaging Techniques
Spring 2005
Techniques Included in the Course
  1. Basic Digital Photography
  2. Electronic Acquisition of Microscopic Images
    1. Light Microscopy  (microscope primer)
      1. General Concepts
      2. Anatomy
      3. Brightfield
        1. upright
          1. AX70 Protocol
          2. Image Capture with the Olympus DP70 Digital Camera
        2. inverted
          1. IX70 Protocol
        3. stereo
          1. Protocol
          2. Image Capture with the Fujifilm Digital Camera
      4. Phase Contrast
        1. upright
        2. inverted
      5. Differential Interference Contrast
        1. AX70
      6. Fluorescence microscopy (concepts)
        1. AX70
        2. Fluorochrome Concepts from Molecular Expressions Microscopy Primer
        3. Table of Fluorochromes
    2. Electron microscopy (demonstration only)
      1. Transmission EM
      2. Scanning EM
      3. Flow Cytometry (demonstration only)
    3. Editing Images with Adobe Photoshop
  3. Preparation of Tissue Sections on Microscope Slides
    1. Paraffin Techniques (overview)
      1. Fixation/Embedment
      2. Sectioning
      3. Staining
        1. Routine
        2. Cytochemical
        3. Immunocytochemical
        4. In situ Hybridization
    2. Plastic Techniques
      1. Fixation/Embeddment & Overview
      2. Breaking Glass Knives
      3. Sectioning
      4. Routine Staining
  4. Image Analysis (with Image Processing Toolkit)
  5. Image Documentation
    1. Computer Printers
    2. Web Page
Last Update: 11-Jan-05
Published by Department of Biology & Microbiology, South Dakota State University
E-mail: michael.hildreth@sdstate.edu