SDSU BIOSTRESS MicroImaging Lab (Part of the Functional Genomics Center)

Overview:

The SDSU Biostress MicroImaging Lab is a multiuse facility that can be used by all SDSU faculty and students following a short orientation session.  First time users first-time users should contact Dr. Mike Hildreth or Dong He for orientation to the facility.

Mike Hildreth michael.hildreth@sdstate.edu 688-4562

Dong He dong.he@sdstate.edu 688-4808

The facility is located in the Northern Plains Biostress Laboratory. It is equipped with three research grade Olympus microscopes (a SZH10 stereo-microscope, an IX70 inverted-microscope and a AX70 compound microscope), PC computers for image manipulation and analysis, and a copy stand for macro-imaging.  These instruments provide fluorescent and white light capability for imaging biological specimens at magnifications from 2.5 to 1000 X.  An Olympus Fluoview FV300 Laser Scanning Confocal Microscope System will soon be interfaced with the IX70 Microscope.

§         Olympus AX70 Upright Compound Microscope Equipped for Epi-fluorescence and DIC Microscopy

§         Olympus IX70 Inverted-Microscope Equipped for Epi-fluorescence and Phase-contrast Microscopy

§         Olympus Fluoview FV300 Laser Scanning Confocal Microscope System Interfaced with the IX70 Microscope

§         Olympus SZH10 Stereo-Microscope equipped with a Fujifilm Digital Camera

§         Olympus DP70 Digital Camera that Interfaces with the AX70 and IX70 Microscopes

Laboratory Resources:

1. Basic Digital Photography
2. Electronic Acquisition of Microscopic Images
1. Light Microscopy  (microscope primer)
1. General Concepts
2. Anatomy
3. Brightfield
1. upright
1. AX70 Protocol
2. Image Capture with the Fujifilm Digital Camera
3. Image Capture with the Olympus DP70 Digital Camera
2. inverted
1. IX70 Protocol
3. stereo
1. Protocol
2. Image Capture with the Fujifilm Digital Camera
4. Phase Contrast
1. upright
2. inverted
5. Differential Interference Contrast
1. AX70
6. Fluorescence microscopy (concepts)
1. AX70
2. Fluorochrome Concepts from Molecular Expressions Microscopy Primer
3. Table of Fluorochromes
2. Electron microscopy (demonstration only)
1. Transmission EM
2. Scanning EM
3. Flow Cytometry (demonstration only)
3. Editing Images with Adobe Photoshop
3. Preparation of Tissue Sections on Microscope Slides
1. Paraffin Techniques (overview)
1. Fixation/Embedment
2. Sectioning
3. Staining
1. Routine
2. Cytochemical
3. Immunocytochemical
4. In situ Hybridization
2. Plastic Techniques
1. Fixation/Embeddment & Overview
2. Breaking Glass Knives
3. Sectioning
4. Routine Staining
4. Image Analysis (with Image Processing Toolkit)
5. Image Documentation
1. Computer Printers
2. Web Page