PHOTOGRAPHY
USING THE OLYMPUS DP70 DIGITAL CAMERA
http://www.olympusmicro.com/primer/java/digitalcameras/olympusdp70/
Labeled Components
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Make sure that you have optimized the
image as described elsewhere in this website.
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Verify that the DP70 Digital Camera is properly attached to either Port
1 or Port 2
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The camera automatically turns on when the DP Controller Program is initiated
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The computer is probably already running and you can wake it up by moving
the mouse or tapping a key on the keyboard
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You will then see the following screen
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Select Imager, and you will be taken immediately to the desktop shown below:
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Activate the DP Controller Program at Clicking on that Icon; you show then
see the screen shown below
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While looking through the microscope oculars, find the area of interest
on the specimen slide, and make sure that the image is in clear focus through
the oculars
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Select the appropriate port (either Port 1 or Port 2) using the Light
Path Selector Button
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To Turn on the camera and to start capturing the images, click on the "eyeball
icon" shown below
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Once the Image Record Window appears on the computer monitor (see below),
you should be able to see the microscope image in the Preview Screen in
the Window.
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You should be able to frame up and focus the microscope image using the
Preview Screen. In addition to the specimen, you will also notice
a yellow hollow box in the middle of the preview area. This box defines
the spot size. The spot size is the area of the preview screen that
is used to automatically calculate and adjust the expose time for that
photograph. When you see the spot size box, you know that you are in the
"Auto" Exposure Mode. In this mode you can drag the spot size box
around to cover the area of the screen that is the primary interest for
the photograph. The default for this setting is 30% of the total
preview screen, but you can change it to either 1% or 0.1% in the spot
box seen below.
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For conventional white-light photographs, you can either allow the camera
determine the exposure settings (Auto Exposure Mode) or you can manually
select the settings (Manual Exposure Mode). The SFLAuto setting is
designed for fluorescence photography.
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If you select the manual exposure mode, you will then be able to manually
adjust the exposure time using the slider feature for exposure time.
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You can adjust the still image size by selecting down arrow in that window
(see below). Under most applications, you will likely want to select
the image size involving the highest number of pixels (4080X3072)
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The defaults for Sensitive (ISO200), Shift number (9), and count (1) should
be function well for most applications.
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Next, you will need to adjust the color balance. To do this, you
will need to click on the Color Balance tab shown above.
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For conventional white-light photographs, you will want to adjust the white
balance; for fluorescence photography, you will need to adjust the black
balance (after selecting the SFL Exposre Mode in the Capture tab).
To adjust either the white or black balance, you will need to turn the
balance on and then click on the "One Touch" button. A eye-dropper
tool will replace the normal cursor. You will then need to move the
eye-dropper tool into the preview screen and click on an area of the screen
that contains white pixels. The preview screen should then shift
of a proper color balance as shown below. If the proper color balance
is not achieved and an error message is given, then the voltage of the
microscope light-sources may be set too low.
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If the image in the preview screen look appropriate at this point, then
the image can now be recorded, if not, additional adjustments must be made.
To record the image, you will need to return to the capture control tab.
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Then, you will need to click on the Camera Icon. After several seconds,
the Edit/Save Window will replace the Image Record Window, and the image
will appear in that window (as shown below)
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If the if appears appropriate, then you can save the image by selecting
file/save in the top menu bar.
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The image can be temporarily saved on the D-drive until it can be moved
to a Zip Disk or burned on a CD
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When you are ready to quit, you must exist out of DP Controller, but you
do not need to turn off the camera, monitor or computer