2001 SURVEY FOR MOSQUITOES IN EASTERN SOUTH DAKOTA
In 2001, the South Dakota Department of Health initiated a program to monitor mosquitoes in South Dakota for the presence of the West Nile Virus.  During the first year (2001), a pilot survey was conducted at four sites (Brandon, Brookings, Castlewood, and Watertown) in eastern South Dakota under the direction of Drs. M.B. Hildreth (Department of Biology/Microbiology and M.A. Catangui (Department of Plant Science) at South Dakota State University.  Two SDSU undergraduate students, Jeff Nolz and Kayla Zirpel (Department of Biology/Microbiology), performed the collections and identified the mosquitoes to the the species level.  Dr. Paul Johnson (SDSU, Department of Plant Science) assisted in some of the mosquito identifications and provided comparative species from the South Dakota Entomological Museum.  Several South Dakota residents (Ms Peg Holmquest, Ms Donna Juffer, Mr. & Mrs. Terry Drew) assisted in the collection of mosquitoes.  This pilot survey focused on the eastern part of the state because if the West Nile Virus is ever found in South Dakota, it will likely appear in the eastern side first.  To collect mosquitoes, CDC miniture light traps (J.W. Hock Inc., Gainesville, FL) were used without carbon-dioxide baiting, beginning on July 3 and ending on August 2, 2001.  The light traps were equipped with an internal programmable photo-switch such that the light and fan is turned on at dusk and and then off at dawn.  The traps were checked each morning and the mosquitoes were immediately frozen in  conventional freezers (approximately -20C).  During the same week, the mosquitoes were thawed briefly, identified and then re-frozen at -70C.   Frozen mosquiotes were sent to the S.D. Department of Health on dry ice to be tested for the presence of the virus.  During this pilot study, a total of 2,045 mosquitoes were collected.  Only the results from Brookings and Watertown are shown below.  Results from this small collection showed that the most prominent species of mosquitoes in eastern South Dakota were Aedes vexans (88.2%), Culex tarsalis, (5.2%), and Aedes dorsalis (4.9%).  Less than 1% of the total mosquitoes were Culex salinarius and Culiseta inornata.    West Nile Virus was not identified in any of the mosquitoes tested.

Collection Sites
  Satellite image provided by the USGS EROS Data Center.

Results from each collection site:

Brookings

Watertown
 

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